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Techniki biologii molekularnej

Techniki biologii molekularnej

Beatriz Dantas Guimarães

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Editorial:
KS OmniScriptum Publishing
Año de edición:
2024
Materia
Genética (no médica)
ISBN:
9786208515508
41,38 €
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Reakcja łańcuchowa polimerazy (PCR) ma jako główne zastosowanie możliwość amplifikacji odcinka DNA, wytwarzając miliony kopii wybranego odcinka. Klonowanie genów ma na celu wprowadzenie wybranego genu do bakterii, które są identyczne ze sobą, a technika ta wymaga obecności wektora klonującego zdolnego do amplifikacji sklonowanego fragmentu DNA. Gen E wirusa dengi (DENV) jest najczęściej wykorzystywany w badaniach molekularnych ze względu na znaczenie białka E. Celem tego badania jest ustalenie technik amplifikacji genu E wirusów DENV-2 i DENV-4. Cząsteczka DNA została uzyskana z FIOCRUZ w Recife; startery zostały wybrane tak, aby zawierały gen E. Fragmenty zostały rozdzielone za pomocą elektroforezy w żelu agarozowym. Amplifikacja PCR została zadowalająco ustalona dla genu E. Po ustaleniu, techniki te są niezbędne na etapach uzyskiwania rekombinowanego DNA w procesie klonowania i ekspresji białka E dengi.

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    Die Polymerase-Kettenreaktion (PCR) dient in erster Linie der Vervielfältigung eines DNA-Abschnitts, wobei Millionen von Kopien des ausgewählten Abschnitts entstehen. Das Klonen von Genen zielt darauf ab, ein ausgewähltes Gen in Bakterien einzuführen, die untereinander identisch sind, und diese Technik erfordert das Vorhandensein eines Klonierungsvektors, der in der Lage ist, e...
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