High resolution melting assay for detection STD bacterial infections

High resolution melting assay for detection STD bacterial infections

Kais Ghaima / Riyam Basim

96,20 €
IVA incluido
Disponible
Editorial:
KS OmniScriptum Publishing
Año de edición:
2025
Materia
Genética (no médica)
ISBN:
9786208425425
96,20 €
IVA incluido
Disponible
Añadir a favoritos

The current study was conducted to detect sexually transmitted infectious agents (bacteria and viruses) by developing HRM ( high resolution melting) assay with multiplex real-time PCR using of set primer of each pathogen that was designated by professional software program (Beacon Designer™ automates the design of real time primers and probes) for the accurate detection of these infections in Iraqi patients. This study conducted for detection seven different sexually transmitted pathogens (3 bacteria, 3 viruses, and one parasite) among 150 suspected patients as married couples. The results of RT-PCR with HRM assay of the positive controls demonstrated different melting curves, where each melting peak represent the amlification of one of the seven pathogens, also the melting peak of the internal control (housekeeping gene). This experiment included three panels, the panel one represents the optimal melting peak for the positive control of Trichomonas vaginalis, Mycoplasma hominis, Cytomegalovirus the melting temperature each of them was 80.0℃, 84.90℃ and 88.0℃ respectively.

Artículos relacionados

  • PCR Troubleshooting and Optimization
    Suzanne Kennedy
    The polymerase chain reaction (PCR) is a fundamental tool in scientific research and clinical testing. Real-time PCR, combining both amplification and detection in one instrument, is a rapid and accurate method for nucleic acid detection and quantification. Although PCR is a very powerful technique, the results achieved are valid only if the appropriate controls have been emplo...
  • BIOCOMPUTING 2018
    A KEITH DUNKER LAWRENCE RUSS B ALTMAN
     The Pacific Symposium on Biocomputing (PSB) 2018 is an international, multidisciplinary conference for the presentation and discussion of current research in the theory and application of computational methods in problems of biological significance. Presentations are rigorously peer reviewed and are published in an archival proceedings volume. PSB 2018 will be held on January ...
    Disponible

    287,37 €

  • Quantitative Real-Time PCR in Applied Microbiology
    Filion
    Real time quantitative PCR (qPCR) technology has revolutionized almost all areas of microbiology, including clinical microbiology, food microbiology, industrial microbiology, environmental microbiology, and microbial biotechnology. Various modifications and improvements have enhanced the overall performance of this highly versatile technology and the qPCR instrumentation and st...
  • BIOCOMPUTING 2016 - PROCEEDINGS OF THE PACIFIC SYMPOSIUM
    A KEITH DUNKER LAWRENCE RUSS B ALTMAN
     The Pacific Symposium on Biocomputing (PSB) 2016 is an international, multidisciplinary conference for the presentation and discussion of current research in the theory and application of computational methods in problems of biological significance. Presentations are rigorously peer reviewed and are published in an archival proceedings volume. PSB 2016 will be held on January ...
    Disponible

    267,56 €

  • Tratamento Avançado para Síndrome de Marfan
    Josias Monias Xavier
    A Síndrome de Marfan é uma condição complexa que desafia a compreensão convencional da medicina. Neste livro, o autor nos leva a uma jornada fascinante pelo mundo da Apometria, Programação Neurolinguística (PNL), Regressão e Hipnose, explorando abordagens alternativas e holísticas para o tratamento desta síndrome e outras condições genéticas. É uma obra que desafia os limites d...
  • Molecular Biology
    G.P. JEYANTHI
    This book delves deep into the fascinating world of molecular biology, a term first coined by William Astbury in 1945. This comprehensive book serves as an authoritative guide to the chemical and physical structures of biological macromolecules and the intricate world of genes. It offers a panoramic view of molecular biology, weaving together genetics, biochemistry, cell biolog...
    Disponible

    32,34 €