Du sang à l’ADN

Du sang à l’ADN

PhD Mustafa Kamal / Shezaib Siddiqui

48,57 €
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Editorial:
KS OmniScriptum Publishing
Año de edición:
2024
Materia
Genética (no médica)
ISBN:
9786207718948
48,57 €
IVA incluido
Disponible
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L’extraction de l’ADN du sang est une étape essentielle pour divers processus en aval qui comprennent le dépistage parental, le séquençage de l’ADN, le clonage de gènes et d’autres méthodes de diagnostic et de recherche. L’ouvrage porte sur l’optimisation d’une méthode rentable d’extraction de l’ADN à partir d’échantillons de sang total humain. Dans cette étude, le protocole original du légendaire scientifique Friedrich Miescher a été modifié de manière à réduire considérablement les délais et les coûts. L’étude a prouvé avec succès que l’ADN du parasite du sang (Plasmodium) peut également être isolé, comme le montre la détection par PCR du parasite dans les échantillons d’ADN extraits de patients infectés. Le protocole défini dans cette étude peut être facilement adopté par n’importe quel laboratoire de biologie moléculaire et, en raison de son faible coût et des exigences techniques, des ressources limitées et du faible budget, les chercheurs peuvent facilement le reproduire en laboratoire.

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